Nucleic Acids Res. Acad. This proliferation of conditional animal models attests to the value of the recombinases as a molecular switch.Another interesting application of gene targeting is knock-in technology, in which any gene of interest can be placed under the cis-acting regulatory elements of another gene (Cohen-Tannoudji and Babinet, 1998). The 5’ arm, in this case, will typically contain homologous genomic sequence that is upstream from the protein start site. Sci.
Long targeting arms do no increase the efficiency of homologous recombination in the β-globin locus of murine embryonic stem cells. Deletion of a DNA polymerase β gene segment in T cells using cell type-specific gene targeting. With negative selection, the HSV thymidine kinase (HSV-tk) gene is often used to select against random integrants. [PubMed]Torres RM, Khn R. 1987;51:503512. Protein domains can be switched by homologous recombination as well. 1994;14:10091016. During homologous recombination, a single copy of a transgene is carried into the restored hprt genetic locus. This solution is then heated at 95C for 15 minutes and then cooled to room temperature.
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